Recognition of the blood group H type 2 trisaccharide epitope by 28 monoclonal antibodies and three lectins |
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| Authors: | Rosella Mollicone Anne Cailleau Anne Imberty Pierre Gane Serge Perez Rafael Oriol |
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| Institution: | (1) INSERM U.178 and Université Paris-Sud, 16 Avenue Paul Vaillant-Couturier, F94807 Villejuif cedex, France;(2) Laboratoire de Synthèse Organique-CNRS, Faculté des Sciences et Techniques, 2 rue de la Houssinière, F44072 Nantes cedex 03, France;(3) Institut National de la Transfusion Sanguine, 75015 Paris, France;(4) Ingénierie Moléculaire, INRA, BP 1627, F44316 Nantes cedex 03, France |
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| Abstract: | The patterns of cross-reaction of 30 monoclonal antibodies and three lectins were determined by ELISA with 21 ABH, Ii or Lewis related synthetic oligosaccharides coupled to bovine serum albumin. At least seven main groups of cross-reactive patterns were identified among the antibodies, plus several intermediate patterns between two of the main antibody groups. The three lectins had different cross-reaction patterns,Galactia tenuiflora was different from all the antibodies,Ulex europaeus lectin 1 andLotus tetragonolobus were similar, but not identical to groups III and V of antibodies respectively. The anti-H antibodies cross-reacting with A type 2 gave similar agglutination scores with all the normal ABO erythrocytes, while the anti-H antibodies not cross-reacting with A type 2 reacted with different scores: O>A2>A2B>B>A1>A1B>Oh, suggesting that these antibodies react better with the free H epitopes and do not recognize the H in A or B epitopes. Based on the ELISA and agglutination results and the lowest energy conformations of each oligosaccharide obtained by computer modelling, the most probable oligosaccharide surface areas recognized by each antibody main group are illustrated. |
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| Keywords: | ELISA synthetic oligosaccharides monoclonal antibodies blood group H lectins ABO |
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