沼泽红假单胞菌J001产辅酶Q10摇瓶发酵条件优化

本试验对沼泽红假单胞菌J001菌株250ml摇瓶发酵辅酶Q10条件进行了优化。结果表明其最佳初始pH值为6.5-7.5，温度为28-31℃，摇床转速100 r min-1，摇瓶装液量为200ml，接种量达10%时可直接进入对数生长期；碳源以NaAc较好，氮源以(NH4)2SO4和NH4Cl较好，磷源用量对考察指标影响不明显；用中心组合设计响应曲面法对碳氮源用量进行了优化，当NaAc浓度为5.39（g l-1），(NH4)2SO4浓度为0.385（g l-1）即碳氮比为14/1时，对菌胞生长最有利；当NaAc浓度为5.70（g l-1），(NH4)2SO4浓度为0.365（g l-1）即碳氮比为15.6/1时，对辅酶Q10产量最有利。

Optimization of shaking-flask culture conditions for CoQ10 production by Rhodopseudomonas palutris J001

The culture conditions in 250 ml flask for the production of CoQ10 by Rhodopseudomonas palutris J001 were investigated. The results showed that the optimal initial pH, temperature, rpm of rotary shaker, medium volume in the flask and inoculum’s size were 6.5-7.5, 28-31℃, 100 rev min-1, 200 ml and 10%, respectively. The suitable carbon source was NaAc and suitable nitrogen sources were (NH4)2SO4 and NH4Cl. But the effects of phosphorus source on the cell growth and CoQ10 production were insignificant. With the aid of response surface method, the optimum concentrations of NaAc and (NH4)2SO4 were 5.39（g l-1）and 0.385（g l-1）for the growth of biomass, respectively, which the ratio of C/N was 14/1; and the optimum concentrations of NaAc and (NH4)2SO4 were 5.70（g l-1）and 0.365（g l-1）for the production of CoQ10, respectively, which the ratio of C/N was 15.6/1.