Adult hematopoietic progenitors are multipotent in chimeric mice |
| |
Authors: | Bernard Pessac Vamshi K Nimmagadda Tapas Makar Paul S Fishman Christopher T Bever David Trisler |
| |
Affiliation: | CNRS UMR 8118, Université Paris Descartes, 45, rue des Saints-Pères, 75006 Paris, France. |
| |
Abstract: | Embryonic stem cells (ESCs) and adult somatic cells, induced to pluripotency (iPSCs), can differentiate into multiple cell lineages. We previously reported that adult mammalian bone marrow contains a sub-population of CD34+ cells that express genes of ESCs and genes required to generate iPSCs. They also express lineage genes of the three embryonic germ layers. Are these CD34+ cells multipotent? Here, CD34+ bone marrow stem cells from adult male ROSA mice, which carry two markers: the β-galactosidase gene and the male Y chromosome, were transplanted into blastocysts of wildtype mice. Each female ROSA chimera generated had a distinct pattern of male-derived organs expressing β-galactosidase; e.g., ectodermal brain, dorsal root ganglia and skin; mesodermal heart, bone and bone marrow; and endodermal pancreas, intestine, and liver. Thus, adult mammals carry cells that appear to exhibit a developmental potential reminiscent of ESCs and iPSCs suggesting they could be used for cell replacement therapy. |
| |
Keywords: | Stem cells Cell therapy Tissue repair Bone marrow Brain Pancreas Heart |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|