Enzymatic deacylations of esterified saccharides--III. Comparison of de-esterifications by serum esterases from different sources |
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| Authors: | S Tomi? L Sesarti? J Tomasi? |
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| Institution: | Department of Radioimmunology, Institute of Immunology, Zagreb, Yugoslavia. |
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| Abstract: | 1. 14C-labelled methyl 2,6-di-O-pivaloyl-alpha-D-glucopyranoside (1) was used as a substrate for esterases from rabbit, guinea pig, mouse, donkey, pig, horse, sheep and human sera. 2. Stepwise de-esterification of the diester substrate 1 occurred with rabbit, guinea pig and mouse serum. Data on time-course experiments and kinetic data are reported. 3. The use of donkey, pig, horse, sheep and human serum led to the migration of the 2-O-pivaloyl group in substrate 1 to the position 4- in the sugar molecule, followed by stepwise de-esterifications of both 1 and the newly formed methyl 4,6-di-O-pivaloyl-alpha-D-glucopyranoside (4). A report is given on the time-course experiments. |
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