Gene expression profiling in porcine mammary gland during lactation and identification of breed-and developmental-stage-specific genes |
| |
Authors: | Zhixi Su Xinjiao Dong Bing Zhang Yanwu Zeng Yan Fu Jun Yu Songnian Hu |
| |
Institution: | (1) James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou, 310008, China;(2) School of Life and Environmental Sciences, Wenzhou Normal College, Wenzhou, 325027, China;(3) Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 101300, China |
| |
Abstract: | A total of 28941 ESTs were sequenced from five 5′-directed non-normalized cDNA libraries, which were assembled into 2212 contigs and 5642 singlets using CAP3. These sequences were annotated and clustered into 6857 unique genes, 2072 of which having no functional annotations were considered as novel genes. These genes were further classified into Gene Ontology categories. By comparing the expression profiles, we identified some breed-and developmental-stage-specific gene groups. These genes may be relative to reproductive performance or play important roles in milk synthesis, secretion and mammary involution. The unknown EST sequences and expression profiles at different developmental stages and breeds are very important resources for further research. |
| |
Keywords: | expressed sequence tags expression profile domestic pig mammary gland |
本文献已被 万方数据 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学:生命科学英文版》浏览原始摘要信息 |
| 点击此处可从《中国科学:生命科学英文版》下载免费的PDF全文 |
|