Transposition pattern of a modified Ds element in tomato |
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Authors: | Caius M T Rommens Tichafa R I Munyikwa Bert Overduin H John J Nijkamp Jacques Hille |
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Institution: | (1) Department of Genetics, Free University, De Boelelaan 1087, 1081 HV Amsterdam, Netherlands;(2) Present address: Department of Plant Pathology, University of California, Berkeley, CA 94720, USA |
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Abstract: | Several aspects of transposition of an in vitro modified Ds element are described. This Ds element, designated ds-r, is equipped with bacterial plasmid sequences and can, therefore, be rescued from the plant genome. Our results indicate that the Ds-r element has a late timing of transposition from T-DNAs. This feature of the element might be advantageous for tagging experiments because it leads to independently transposed germinally transmitted elements. Furthermore, it is shown that Ds-r transposition generates clusters of insertions, indicating that genes to be tagged should be located in genomic regions covered by insertions. |
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Keywords: | transposition Ac/Ds transgenic tomato plants RFLP mapping plasmid rescue |
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