Development of a beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis |
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| Authors: | P Haima D van Sinderen H Schotting S Bron G Venema |
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| Affiliation: | Department of Microbiology, Center of Biological Sciences, Haren, The Netherlands. |
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| Abstract: | A versatile beta-galactosidase alpha-complementation system for Bacillus subtilis was developed, which can be used for molecular cloning in this Gram+ organism. The cloning system, which is based on the highly efficient host-vector system 6GM-pHP13, offers several advantages over previously described systems: (1) convenient direct selection of recombinants; (2) the cloning of large heterologous DNA fragments with high efficiency; and (3) the availability of six unique target sites: SphI, NdeI, NheI, BamHI, SmaI and EcoRI. |
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