| 干旱胁迫对蝉拟青霉SOD活性的影响 |
| |
| 引用本文： | 胡海燕,邹晓,王鹏霞,刘爱英,梁宗琦.干旱胁迫对蝉拟青霉SOD活性的影响[J].菌物研究,2008,6(1):54-56. |
| |
| 作者姓名： | 胡海燕 邹晓 王鹏霞 刘爱英 梁宗琦 |
| |
| 作者单位： | 贵州大学生命科学学院真菌资源研究所,贵阳,550025 |
| |
| 摘 要： | 为探讨蝉拟青霉Paecilomyces cicadae抗旱的生理生化基础,利用聚乙二醇6000(PEG-6000)对采自贵州省荔波县的蝉拟青霉GZUIFR-3L菌株液体发酵进行胁迫,用氮蓝四唑法连续测定不同培养时间SOD活性,考马斯亮蓝G-250法测定蛋白质含量,从而测定SOD比活力。结果表明,胁迫和非胁迫情况下,蝉拟青霉蛋白质含量、SOD活性和SOD比活力分别在发酵培养第7,8,6d时达到最大值;经干旱胁迫的蝉拟青霉SOD活力及其比活力均比未经胁迫处理的高,初步推测蝉拟青霉抗旱能力与SOD活性有一定的相关性。
|
| 关 键 词： | 蝉拟青霉 干旱胁迫 聚乙二醇-6000 SOD |
| 文章编号： | 1672-3538(2008)01-0054-03 |
| 修稿时间： | 2007年10月6日 |
Effect of Drought Stress on SOD Activity of Paecilomyces cicadae |
| |
| HU Hai-yan,ZOU Xiao,WANG Peng-xia,LIU Ai-ying,LIANG Zong-qi.Effect of Drought Stress on SOD Activity of Paecilomyces cicadae[J].Journal of Fungal Research,2008,6(1):54-56. |
| |
| Authors: | HU Hai-yan ZOU Xiao WANG Peng-xia LIU Ai-ying LIANG Zong-qi |
| |
| Institution: | ( Institute of Fungus Resources, College of Life Science, Guizhou University, Guiyang 550025, China) |
| |
| Abstract: | The Paecilomyces cicadae strain GZUIFR - 3L , collected from Libo county, Guizhou province, was studied. It was cultured under drought stress by PEG - 6000. Their SOD activities and protein contents in different times were respectively determined with the NBT- illumination method and coomassie brilliant blue G250 method. Then the specific activities were worked out. The results showed that protein contents, SOD activities and SOD specific activities had the maximum values respectively on the 7th,Sth,6th days. The results also showed that the P. cicadae had higher protein contents, SOD activities and SOD specific activities when they were under drought stress than in normal condition. It followed that the SOD activity was likely related to some extent to the drought-resistant ability. |
| |
| Keywords: | Paecilomyces cicadae droughtstress PEG - 6000 SOD |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
