| 固相滤纸法测定鼠肝糖原合成酶的活性 |
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| 引用本文： | 王艳荣,钱荣立,贺师鹏,黄天贵. 固相滤纸法测定鼠肝糖原合成酶的活性[J]. 生物化学与生物物理进展, 1997, 24(6): 554-556 |
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| 作者姓名： | 王艳荣 钱荣立 贺师鹏 黄天贵 |
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| 作者单位： | 北京医科大学第一医院，北京 100034;北京医科大学第一医院，北京 100034;北京医科大学生物物理系，北京 100083;北京医科大学生物物理系，北京 100083 |
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| 基金项目： | 国家教委回国人员启动基金. |
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| 摘 要： | 探求简捷有效的测定鼠肝糖原合成酶活性的方法. 固相滤纸法测定该酶活性，并与文献报告的漂洗法进行比较，且用此方法观察糖尿病鼠肝糖原合成酶的活性. 两种方法所测得放射性计数无明显差异；糖尿病鼠肝糖原合成酶的活性明显降低. 固相滤纸法是一种较简捷，方便，不易污染的测定糖原合成酶的方法.
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| 关 键 词： | 糖尿病，滤纸法，糖原合成酶 |
| 收稿时间： | 1996-12-20 |
| 修稿时间： | 1997-04-30 |
A Solid Phase Filter Paper Method for the Assay of Glycogen Synthase Activity in Rat Liver |
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| WANG Yanrong,QIAN Rongli,HE Shipeng and HUANG Tiangui. A Solid Phase Filter Paper Method for the Assay of Glycogen Synthase Activity in Rat Liver[J]. Progress In Biochemistry and Biophysics, 1997, 24(6): 554-556 |
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| Authors: | WANG Yanrong QIAN Rongli HE Shipeng HUANG Tiangui |
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| Affiliation: | Department of endocrinology of the first hospital of Beijing Medical University, Beijing 100034, China;Department of endocrinology of the first hospital of Beijing Medical University, Beijing 100034, China;Department of Biophysics of Beijing Medical University, Beijing 100083, China;Department of Biophysics of Beijing Medical University, Beijing 100083, China |
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| Abstract: | A method improved for the assay of glycogen synthase(GS) is proposed in the present study. GS activity in NIDDM rat liver is determined by the solid phase filter paper method in comparison with washing method reported in the literatures. There is no obvious difference in the determination of radiation counts between the two methods. This method has been employed in the determination of GS activity of the diabetic rat liver, which exhibited more decrease then the normal rat liver. Therefore, the solid phase filter paper method is simple, convenient for the assay of GS activity and shows no tendency to be polluted. |
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| Keywords: | diabetes mellitus filter paper method glycogen synthase |
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