柑桔碎叶病毒研究

用汁液摩擦接种方法对柑桔碎叶病毒(Citrus tatter leaf virus,CTLV)进行了进一步的生物学鉴定.结果表明,该病毒除在豇豆上引起枯斑外,还侵染克里芙兰烟(Nicotianaclevilandii)产生系统斑驳和轻花叶,侵染昆诺藜(Chenopodium quinoa)和苋色藜(C.amaranticolor)引起系统坏死和花叶,侵染鸡冠花(Celosia cristata)引起局部环斑.这些草本寄主均可作为CTLV的指示植物.在发病的柑桔叶汁液中,测得CTLV的稀释限点为10~(-5).经汁液摩擦接种,可以将CTLV从克里芙兰烟传播到木本指示植物柑桔和枳橙上.感病植物材料的组织超微结构观察结果表明:CTLV感染柑桔、枳橙、克里芙兰烟和昆诺藜均使其叶肉薄壁细胞的叶绿体出现淀粉沉积、叶绿体片层结构解体和消失;病毒在受感染的植株叶脉韧皮部细胞中紧密聚集,形成病毒结晶体;这种结构也出现在叶肉薄壁细胞中.这是关于该病毒组织病变的首次报道.用直接负染方法从感染CTLV的柑桔(枳橙)、克里芙兰烟和昆诺藜病叶中均能检查到线状病毒粒子,用改良的Deriick’s免疫电镜方法和琼脂双扩散方法测定均显示该病毒…

A STUDY ON CITRUS TATTER LEAF VIRUS

Citrus tatter leaf virus(CTLV)was mechanically transmitted to 23 herbaceousspecies from eight families. It induced local lesion on Vigna sinensis, systemic yellow spotand distortion on Chenopodium quinoa and C. amaranticolor. local spot or Celosia cristataand systemic light mottle on Nicotiana celevilandii. It was also back transmitted fromN. celevilandii to Citrus reficulata,C, citrange and caused typical "tatter leaf "symptoms.DEP for this virus was 10-5, when tested from infected leaf sap of C. reticulata. Virusparticles that characterized as Capiloviruses were detected under electron microscopefrom leaf-sap of diseased hosts above. Herbaceous species can be used as indicators forthis virus. Virus crystallized bodies were found in phloem cells of C. reticulata anc C.citrange infected with CTLV, the same structures were also observed in mesophyll cellsof Chenopodium quinoa and N. clevilandii infected with CTLV. but the crystallizationbodies were looser aggregated and smaller. Degradation of reticulum and accumulating ofstarch bodies was observed on chloroplasts of infected mesophyll cells of above hosts.This is the first report of cytopathological characteristics of this virus disease. Positiveserological relationship was found between CTLV and apple stem grooving virus( ASGV) by agar double diffusion test and ISEM. This indicated the possibility for usingASGV antibodies to detect CTLV in plant materials.