PHB颗粒在红豆草根瘤细菌发育中的动态变化

红豆草根瘤胞间隙和侵入线中另有个别细菌含有PHB颗粒,而且数量很少,一个细菌通常仅有一个。随着细菌被从侵入线中释放到寄主细胞中,这些PHB颗粒立即消失。幼龄细菌不含PHB颗粒,成熟细菌一般也不含这种内含物。当细菌衰老时,它们又再度出现,并大量增加,而后很快减少,直至完全消失。从未发现这种颗粒存在于解体细菌中,尽管它们处于各种不同的解体状态。PHB颗粒在细菌发育中的变化表明,它的多少不仅与根瘤细菌发育密切有关,而且也受制于根瘤品种。

CHANGES OF PHB GRANULES DURING THE BACTEROIDAL DEVELOPMENT IN ONOBRYCHIS VICIAEFOLIA NODULES

Only very few bacteria contain PHB granules in the intercellular spaces and the infection threads of Onobrychis viciaefolia nodules and usually is one in a bacterium. After the bacteria are released from their infection threads to host cells, the PHB granules disappear, The young bacteroids in infected cells do not contain PHB granule, the mature bacteroids in infected usually also do not contain PHB granule, when the bacteroids start senescence, PHB granules appear in the bacteroids again and quickly increase, sometimes they even reach to more than 20, and they can fuse each other and form some larger irregular electron transparent spaces. Then the PHB granules rapidly discrease until they completely disappear. We never find that the PHB granule appear in the disintegrated bacteroids which may be different in disintegration degrees. The changes of PHB granules during the development of the bacteroids in Onobrychis viciaefolia nodules show the PHB granules in the bacteroids are not a constant, on the contrarry, they are not noly close relation with the bacteroidal development in the nodule but also are affected by the nodule veriety.