| 假单胞杆菌2-萘酸加氧酶基因的克隆与表达 |
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| 引用本文： | 孙国萍. 假单胞杆菌2-萘酸加氧酶基因的克隆与表达[J]. 微生物学报, 1995, 35(3): 161-165 |
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| 作者姓名： | 孙国萍 |
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| 作者单位： | 中国科学院武汉病毒研究所 武汉430071(孙国萍)，英国帝国理工与医学学院生物工程中心 伦敦(TheophilouS)，英国帝国理工与医学学院生物工程中心 伦敦(RibbonsDW) |
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| 摘 要： | 采用Cosmid pLARF1构建了完整的2-萘酸代谢菌2-NAT菌株的基因文库.通过分析基因文库中产生蓝色色素的克隆验证了2-萘酸代谢基因在大肠杆菌体内得到表达,并导致重组细菌产生靛蓝.从产生靛蓝的克隆抽提重组的Cosmid DNA进行酶解分析,发现所有能产生靛蓝的克隆均含有一大小相同的DNA片段.用重组细菌进行靛蓝生物合成的试验展示了微生物法生产靛蓝的美好前景.
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| 关 键 词： | 基因文库 加氧酶 假单胞杆菌 2-萘酸 基因克隆 Gene library Indigo Oxygenase |
CLONING AND EXPRESSION OF OXYGENASE GENE FOR 2-NAPHTHOIC ACID FROM PSEUCLOMONAS |
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| Sun Guoping. CLONING AND EXPRESSION OF OXYGENASE GENE FOR 2-NAPHTHOIC ACID FROM PSEUCLOMONAS[J]. Acta microbiologica Sinica, 1995, 35(3): 161-165 |
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| Authors: | Sun Guoping |
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| Abstract: | A gene library which includes total DNA of 2-NAT strain's genenome hasbeen constructed successfully with cosmid pLARFl. The pigment produced by thetransconjugants from the gene library is identified. The result of restriction endonucleasedigestion of the recombinant cosmids purified from indigo producing colonies indicatesthat all of the blue colonies contain a 8. 8kb insert DNA fregment. It shows that theyexpressed the same gene. The indigo biosynthesis experiment with blue colonies revealsa good view of producing the pigment with recombinant microorganism. |
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| Keywords: | Gene library Indigo Oxygenase |
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