赤霉素产生菌——藤仓赤霉菌融合重组的研究

以一对营养互补的缺陷型突变株作为亲本,酶法去除细胞壁制成原生质体,以等量相混,用30%PEG 4000诱导融合,在最低营养再生培养基上直接选择原养型融合重组子,重组频率约为10~-,同时产生一定数量的不稳定异核体,频率约为10~(-5)—10~(-6).融合重组导致色素产生和菌丝形态及赤霉素产生能力的多种变异.融合重组子中赤霉素产量的正变率为15.3%.其中RN2和RG14菌株的赤霉素产量比原养型出发菌株207提高25%以上.

STUDIES ON FUSED RECOMBINATION OF GIBBERELLA FUJIKUR OI-GIBBERELLIN-PRODUCING STRAIN

A pair of nutrient complementary auxotrophic mutants were used as fusion parents. Their protoplasts were prepared enzymatically and PEG4000 was usedas fusion agent. The prototrophic recombinants were selected directly on the minimal regeneration medium. The recombination frequency was about 10-7. Some unstable heterocaryons were occured and the frequency was about 10-5-10-6. Mutation of pigment, hyphae morphology and gibberellin production were induced throughthe fused recombination. The positive mutation, negative mutation of gibberellinyield among the recombinants were 15.3% and 53.1% respectively. Gibberellin yields of recombinants signed RN2 and RG14 were over 25% than that of the prototrophic parent 207 strain.