原生质体融合提高农抗武夷菌素的效价

从农抗武夷菌素产生菌不吸水链霉菌武夷变种菌株Co-N-31诱变获得两个突变株M35(Leu^-,孢子颜色灰色)和M46(ser^-.孢子颜色灰白色),并以此两突变株为直接亲本在25％ PEG1000诱导下进行种内原生质体融合。M35和M46原生质体再生率分别为3.72％和0.248％,重组频率为55.20％。采用间接法选择营养标记互补的稳定的原养型重组子,并从中获得一株高产菌株F31-24;其效价比原始亲本Co-N-31提高了82％。薄层层析结果表明,菌株F31-24和Co-N-31的发效产物在Rf值为0.50和0.26处均有斑点,但含量有异。测定斑点生物活性证明其均有抑菌活性。温室试验表明,菌株F31-24发酵产物对小麦白粉病的防治效果优于菌株Co-N-31。

ENHANCING THE PRODUCTION OF WUYIENCIN THROUGH PROTOPLAST FUSION

Two mutants, M35(Leu , grey spore) and M46 (Ser-, greyish white spore), were obtained from the producing strain of wuyiencin, strain Co-N-31 (Streptomyces ahygroscopicus var. wuyiensis) and intraspecific protoplast fusion was performed between these two mutants with 25% PEG 1000. The regeneration ratio of the protoplasts was 3.72% for strain M35 and 0.248% for strain M46. Stable prototrophic recombinants with frequancy of 55.20% resulted from complementation of nutrient markers of parents were selected indirectly and a highly productive strain F31-24 was obtained from them. The production of F31-24 was 82% higher than that of Co-N-31. The results of TLC indicated that both F31-24 and Co-N-31 had spots at Rf = 0.50 and Rf = 0.26 with different contents. All spots had biological acticities. The experiments in greenhouse showed that the control effect, of F31-24 on wheat powdery mildew was higher than that of Co-N-31.