Gibberellin A12 Is Converted to Gibberellin A53 in Cultured Cells of Nicotiana tabacum and Catharanthus roseus |
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Authors: | Song Nam Hi; Fujioka Shozo; Kamiya Yuji; Sakurai Akira; Liu Jang Ryol |
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Institution: | 1The Institute of Physical and Chemical Research (RIKEN) Wako-shi, Saitama, 351-01 Japan
2Genetic Engineering Research Institute, Korea Institute of Science and Technology Daejon, 305-606 Korea |
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Abstract: | The metabolism of GA12 and its precursors was investigated incultured cells of seven cell lines of Nicotiana tabacum andthree cell lines of Catharanthus roseus using l4C-labeled substrates.The presence of a metabolic pathway from ent-7 -hydroxykaurenoicacid to GA53 via GA12-aldehyde and GA12 was demonstrated inthe cultured cells. GA12 was effectively converted to GA53 incells of BY-2, 2b-4, 2b-13 and CG from N. tabacum. By contrast,GA53 was not converted to any other GAs in all of the linesof cells examined. The metabolism of C19-GAs was also examinedusing 3H-labeled substrates. The conversion of GA20 to GA29and GA, and of GA4 to GA34 occurred more efficiently in cellsfrom C. roseus than in cells from N. tabacum. However, 13-hydroxylationof GA4 and GA9 was not observed in any of the cell culturesexamined. Among the various metabolites, GA53, GA29 and GA34were identified by full-scan GC/MS. (Received December 20, 1990; Accepted May 27, 1991) |
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