Identification of nonsense mutations in Neurospora: Application to the complex arg-6 locus |
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Authors: | Rowland H. Davis and Richard L. Weiss |
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Affiliation: | (1) Department of Molecular Biology and Biochemistry, University of California, Irvine, 92717 Irvine, California, USA;(2) Department of Chemistry and Biochemistry, University of California, Los Angeles, 90024 Los Angeles, California, USA |
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Abstract: | Summary The arg-6 locus of Neurospora crassa encodes two enzymes of arginine synthesis, acetylglutamate kinase and acetylglutamyl phosphate reductase. Mutants lacking one or the other enzyme fall into two different complementation groups; a large non-complementing group lacks both enzymes. We wished to survey over 50 alleles for suppressibility by a nonsense suppressor. We compared two methods of assessing suppressibility. One, based on trans-action of a nonsense suppressor, was simple, but not efficient in detecting all suppressible alleles. The other, based on crosses involving a marker linked to the locus surveyed was very efficient in methodology and is suited to all cases, such as the arg-6 locus, in which allelic crosses are sterile. The data indicate that the arg-6 locus encodes a bifunctional protein. |
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