Hormone-responsive survival of mammary gland explants from the pregnant tammar wallaby (Macropus eugenii) in the absence of exogenous hormones and growth factors. |
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Authors: | K R Nicholas C Collet R Joseph L Sankaran |
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Affiliation: | Division of Wildlife and Ecology, CSIRO, Lyneham, Australia. |
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Abstract: | 1. The level of beta-lactoglobulin mRNA increased maximally in mammary explants from late pregnant tammars cultured for 3 days in media containing either prolactin or insulin, cortisol and prolactin. 2. The same level of accumulation occurred when explants were first cultured for 4 days in a chemically defined medium with no exogenous hormones, serum or growth factors, suggesting that the tissue remains viable and hormone-responsive during the initial incubation. 3. Mammary explants cultured for 4 days in medium with no hormones demonstrated a progressive increase in the rate of RNA and DNA synthesis suggesting that the tissue is under a positive autocrine/paracrine stimulus. |
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