Viability of trisomy 12 cells in mouse chimaeras |
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Authors: | Reinald Fundele Eva-Maria Jägerbauer Ulrike Kolbus Heinz Winking Alfred Gropp |
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Affiliation: | (1) Institut für Pathologie der Medizinischen Hochschule Lübeck, D-2400 Lübeck, Federal Republic of Germany;(2) Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, D-8000 München;(3) Laboratoire de Différenciation Cellulaire, Ecole de Médecine 20, CH-1211 Genève 4, Switzerland |
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Abstract: | Summary Mouse aggregation chimaeras consisting of trisomy 12 and normal euploid cells were produced. The analysis of one trisomy 12euploid chimaera, using biochemical and cytological markers, showed that the trisomic cells were able to participate in the formation of most tissues including the ovary. On the other hand, no trisomy 12 cells were found in lymphocyte populations, which is most likely due to early selection in this particular cell lineage. The viability of two adult trisomy 12 chimaeras demonstrates that trisomy 12 cells are able to develop beyond the fetal stage which is not observed in completely trisomic fetuses.Furthermore, these chimaeras did not show any sign of a trisomy 12 syndrome, indicating that the trisomy 12 cells were functionally integrated and participated normally in the differentiation of the various tissues. Our results suggest that trisomy 12 in the mouse is not autonomously cell lethal but can be rescued and is perfectly viable in the presence of normal diploid cells.This article is dedicated to the memory of Prof. A. Gropp |
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Keywords: | Mouse aggregation chimaeras Trisomy 12 |
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