Stability of plasmid PBR322 in Escherichia coli immobilized on cotton cloth during use as resident inoculum |
| |
Authors: | Sushama Joshi Hiroshi Yamazaki |
| |
Affiliation: | (1) Department of Biology and Institute of Biochemistry Carleton University, K1S 5B6 Ottawa, Canada |
| |
Abstract: | Summary E.coli cells harbouring plasmid pBR322 which confers ampicillin resistance were immobilized on cotton cloth. The resulting film was used as an inoculum in daily repeated batch culture in ampicillin-free medium. During two months, the film was able to produce cultures which, at the late log phase, showed little sensitivity to 10 mg/ml ampicillin. Thus such a bacterial film can effectively be used as an inoculum for the production of recombinant DNA products by means of pBR322 or its derivatives in the absence of ampicillin. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |