Stability of plasmid PBR322 in Escherichia coli immobilized on cotton cloth during use as resident inoculum |
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Authors: | Sushama Joshi Hiroshi Yamazaki |
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Institution: | (1) Department of Biology and Institute of Biochemistry Carleton University, K1S 5B6 Ottawa, Canada |
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Abstract: | Summary
E.
coli cells harbouring plasmid pBR322 which confers ampicillin resistance were immobilized on cotton cloth. The resulting film was used as an inoculum in daily repeated batch culture in ampicillin-free medium. During two months, the film was able to produce cultures which, at the late log phase, showed little sensitivity to 10 mg/ml ampicillin. Thus such a bacterial film can effectively be used as an inoculum for the production of recombinant DNA products by means of pBR322 or its derivatives in the absence of ampicillin. |
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