Attenuation of seizure in P77PMC rats with an HSV-vector expressing IL-1ra in brain |
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Authors: | ZHANG Guorong SHAN Weisong WANG Zhaohui WU Xiru |
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Institution: | (1) Pediatrics Department, The First Teaching Hospital, Beijing Medical University, 100034 Beijing, China |
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Abstract: | Cloned human IL-1ra (IL-1 receptor antagonist, IL-1ra) cDNA is inserted into pHSVLac, resulting in recombinant named pHSV-IL-1ra.
pHSVLac and pHSV-IL-lra were packaged into HSV-1 particles using HSV-1 ts K as helper virus. The results showed that: (i)
vero cell infected by pHSV-IL-1ra particles showed IL-1ra expression; expression of β-galactosidase is observed in meninges
and some neurons closed to ventricular and maintained for 8 weeks in rats after intracerebroventricular injection of pHSV-IL-1ra
particles; (ii) transgenic expression of IL-lra by pHSVIL-lra injection significantly inhibited seizure attacks of P77PMC
rat. These studies indicate that HSV-1 vector expressing IL-1ra in brain attenuated seizure attacks of P77PMC rat.
Project supported by the National Natural Science Foundation of China (Grant No. 39370259). |
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Keywords: | HSV 1 vector IL 1ra seizure P77PMC rat gene transfer |
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