Possible quorum sensing in the rumen microbial community: detection of quorum-sensing signal molecules from rumen bacteria |
| |
Authors: | Mitsumori Makoto Xu Liming Kajikawa Hiroshi Kurihara Mitsunori Tajima Kiyoshi Hai Jin Takenaka Akio |
| |
Institution: | National Institute of Livestock and Grassland Science, Ikenodai 2, Tsukuba, 305-0901, Ibaraki, Japan. mitumori@affrc.go.jp |
| |
Abstract: | The bioluminescence assay using Vibrio harveyi BB170 was used to examine quorum-sensing autoinducer 2 (AI-2) activity from cell-free culture fluids of rumen bacteria. The assay showed that the culture fluids of four species of rumen bacteria, Butyrivibrio fibrisolvens, Eubacterium ruminantium, Ruminococcus flavefaciens, and Succinimonas amylolytica, contained AI-2-like molecules. Furthermore, homologues for luxS genes were detected in rumen fluids collected from three cows and in bacterial cells of P. ruminicola subsp. ruminicola and R. flavefaciens. These findings suggest that the quorum-sensing system mediated by AI-2 is present in the rumen. |
| |
Keywords: | Rumen bacterium Quorum-sensing system Autoinducer 2 activity luxS gene |
本文献已被 PubMed 等数据库收录! |
|