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Average molecular weight and molecular weight distribution of agarose and agarose-type polysaccharides |
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| Authors: | Cyrille Rochas Marc Lahaye |
| Institution: | CERMAV-CNRS, Associé à l'Université J. Fourier, BP 53, 38042, Grenoble, Cedex, France INRA Laboratoire de Biochimie et Technologie des Glucides, Chemin de la Géraudière, BP 527, 44026, Nantes, Cedex 03, France |
| Abstract: | A procedure to determine the absolute weight-average molecular weight and molecular weight distribution of agarose and agarose-type polysaccharides by aqueous size-exclusion chromatography coupled with low-angle laser light scattering is described. The molecular weights of the majority of the commercial samples investigated were between 80 000 and 140 000 with a polydispersity lower than 1·7. In contrast, most of the laboratory-extracted agarose-type polysaccharides had lower molecular weights. |
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