Uptake of horseradish peroxydase by the testis of Locusta migratoria during the last larval instar; relation with variations of ecdysteroid levels in haemolymph

Summary By using horseradish peroxydase (HRP) as a tracer, it is shown that the gonial region of the locust testis is an open compartment which is almost always freely penetrated by the tracer. During the last larval instar, however, the penetration of HRP decreases and ceases at the time when high levels of ecdysteroids are detected in the haemolymph by radioimmunoassay. A cause and effect relationship between tracer uptake and hormonal level could not be demonstrated by the experiments carried out up to now. From ultrastructural observations of the testis, it is concluded that the temporary isolation of the gonial compartment is not based upon any morphological structure which could act as a barrier. Penetration of the macromolecule is considered as the expression of an active uptake by the testis and the short period of nonpenetrability as a state of inertia whose significance remains to be discovered.