Nerve dependent sulphated glycosaminoglycan synthesis in limb regeneration of the newt Pleurodeles waltl |
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Authors: | B Boilly H Hondermarck M Oudghir E Deudon Y Boilly-Marer |
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Institution: | (1) Laboratoire de Biologie Cellulaire et Moléculaire du Développement, Université des Sciences et Technologies de Lille, Lille, France;(2) Laboratoire de Biochimie, Université de Marrakech, Marrakech;(3) Laboratoire de Biochimie INSERM U 402, Faculté de Médicine Saint-Antoine, Paris, France;(4) Université des Sciences et Technologies de Lille, UMR 111, Lille, France |
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Abstract: | Denervation of the amputated limb of newts stops the regeneration process by decreasing blastema cell proliferation. We investigated the effect of the denervation on each of the two compartments (epidermal cap, mesenchyme) in mid-bud blastemas on the level of sulphated glycosaminoglycans (GAGS). Denervation resulted in an increase of about threefold in the incorporation of 35S] sulphate into mesenchyme GAGs but had no effect on the epidermal cap. The increase of GAG synthesis in the mesenchymal part of the blastema involved both heparan sulphates and chondroitin-dermatan sulphates. Gel filtration showed no change in GAGs size after denervation. These results confirm that the mesenchymal part of the mid-bud blastema is the main target of nerves and, as heparan sulphates are known to store acidic fibroblast growth factor (aFGF), a polypeptide found in the blastema (Boilly et al.. 1991), this suggest that the nerves' effect on glycosaminoglycans turnover could be implicated in the control of bioavailability of this growth factor in the blastema. |
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Keywords: | Limb regeneration Denervation Sulphated glycosaminoglycans Heparan sulphates Growth control |
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