Linear correlation between thermal stability and folding kinetics of lysozyme |
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| Authors: | Cao Aoneng Wang Gang Tang Youqi Lai Luhua |
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| Institution: | State Key Laboratory of Structural Chemistry for Stable and Unstable Species, Peking University, Beijing 100871, China. |
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| Abstract: | We have studied the refolding and thermal denaturation of hen egg white lysozyme in a wide range of pH values (from 1.5 to 9.4) using stopped-flow circular dichroism (CD) and differential scanning calorimetry (DSC). A linear correlation was found between the thermal denaturation temperature (T(m)) and the logarithm of the refolding rate of the slow folding phase of hen egg white lysozyme (lnk(2)). |
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| Keywords: | protein folding pH dependence lysozyme stopped-flow circular dichroism differential scanning calorimetry |
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