Phenotypic changes resulting from distinct point mutations in the Azospirillum brasilense glnA gene,encoding glutamine synthetase |
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Authors: | Van Dommelen Anne Keijers Veerle Wollebrants An Vanderleyden Jozef |
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Affiliation: | Center of Microbial and Plant Genetics, Katholieke Universiteit Leuven, B-3001 Heverlee, Belgium. |
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Abstract: | Sequencing the glnA genes of two chemically induced Azospirillum brasilense glutamine synthetase mutants revealed an Arg-->Cys mutation, corresponding to the glutamate binding site, in one mutant and an Asp-->Asn mutation, corresponding to the ammonium binding site, in the second mutant. The phenotypic changes in these mutants are discussed in relation to their genotypes. |
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