Needle in a haystack: Antibacterial activity-guided fractionation of a potato wound tissue extract |
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| Institution: | 1. Department of Chemistry and Biochemistry, The City College of New York, City University of New York and CUNY Institute for Macromolecular Assemblies, New York, NY 10031, USA;2. Department of Chemistry, Hunter College of CUNY, New York, NY 10065, USA;3. Ph.D. Program in Chemistry, The Graduate Center of the City University of New York (CUNY), New York, NY 10016, USA;4. Ph.D. Program in Biochemistry, The Graduate Center of the City University of New York (CUNY), New York, NY 10016, USA;1. Chemistry Department, Faculty of Science, King Khalid University, Abha 61413, Saudi Arabia;2. Chemistry Department, Faculty of Science, Al-Azhar University, Nasr City 11884, Cairo, Egypt;3. Chemistry Department, Faculty of Science, Jazan University, Jazan, Saudi Arabia;4. Pharmacognosy and Pharmaceutical Chemistry Department, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 41477, Saudi Arabia;5. Pharmaceutical Organic Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11884 Nasr City, Cairo, Egypt;6. Biology and Chemistry Department, Al-Qunfudah University College, Umm Al-Qura University, Al-Qunfudah 1109, Saudi Arabia;7. Pharmaceutical Chemistry Department, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia;8. Pharmaceutical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, 11884 Nasr City, Cairo, Egypt;9. Chemistry Department, Faculty of Science and Art, Al-Baha University, Al-Baha 1988, Saudi Arabia;10. Pharmacology and Toxicology Department, College of Pharmacy, Taibah University, Al-Madinah Al-Munawarah 41477, Saudi Arabia;1. Department of Biochemistry, Kangwon National University, Chuncheon 24341, Republic of Korea;2. Proteogen Inc., Seoul, Republic of Korea;3. Shebah Biotech Inc., G-Tech Village, Chuncheon, Republic of Korea;1. Department of Medicinal Chemistry, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China;2. Department of Dermatology, Xiangya Hospital, Central South University, Changsha 410008, China;1. Laboratório de Bioquímica Microbiana, Departamento de Microbiologia Geral, IMPG, Universidade Federal do Rio de Janeiro, CEP 21949-900 Rio de Janeiro, RJ, Brazil;2. Instituto de Química de São Carlos, Universidade de São Paulo, CP 780, CEP 13560-970 São Carlos, SP, Brazil;3. Departamento de Virologia, IMPG, Universidade Federal do Rio de Janeiro, CEP 21949-900 Rio de Janeiro, RJ, Brazil;1. Arizona College of Osteopathic Medicine, Midwestern University, 19555 N. 59th Ave., Glendale, AZ 85308, USA;2. Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;3. Department of Pathology and Population Medicine, College of Veterinary Medicine, Midwestern University, 19555 N. 59th Ave., Glendale, AZ 85308, USA |
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| Abstract: | Erwinia carotovora is a major cause of potato tuber infection, which results in disastrous failures of this important food crop. There is currently no effective antibiotic treatment against E. carotovora. Recently we reported antibacterial assays of wound tissue extracts from four potato cultivars that exhibit a gradient of russeting character, finding the highest potency against this pathogen for a polar extract from the tissue formed immediately after wounding by an Atlantic cultivar. In the current investigation, antibacterial activity-guided fractions of this extract were analyzed by liquid chromatography-mass spectrometry (LC-MS) utilizing a quadrupole-time-of-flight (QTOF) mass spectrometer. The most active chemical compounds identified against E. carotovora were: 6-O-nonyl glucitol, Lyratol C, n-2-(4-Hydroxyphenyl)] ethyldecanamide, α-chaconine and α-solanine. Interactions among the three compounds, ferulic acid, feruloyl putrescine, and α-chaconine, representing metabolite classes upregulated during initial stages of wound healing, were also evaluated, offering possible explanations for the burst in antibacterial activity after tuber wounding and a chemical rationale for the temporal resistance phenomenon. |
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| Keywords: | Potato Wound periderm Antibacterial LC-MS TOF-MS |
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