A selective and sensitive turn-on chemosensor for detection of Fe3+ in aqueous solution and its cell imaging in dorsal root ganglia neurons and MKN-45 cells |
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| Institution: | 1. Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Department of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023,China;2. Department of Biochemical Engineering, Nanjing University of Science and Technology, Chemical Engineering Building B308, 200 Xiaolinwei, Nanjing 210094,China;3. Departments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA;4. College of Science, Nanjing Forestry University, Nanjing 210037, China;1. Research and Development Centre, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India;2. Department of Chemistry, Thiruvalluvar Government Arts College, Rasipuram, 637 401, Tamil Nadu, India;3. Department of Chemistry, Arignar Anna Government Arts College, Namakkal, 637 002, Tamil Nadu, India;1. Department of Applied Chemistry, School of Applied Chemical Engineering, Kyungpook National University, Daegu 41566, Republic of Korea;2. School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea;1. School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers’ University, Yancheng, Jiangsu 224051, People''s Republic of China;2. College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, People''s Republic of China |
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| Abstract: | A new turn-on fluorescent chemosensor (RBTM) for Fe3+ was designed based on Rhodamine B and a thiocarbonylimidazole moiety. The spectroscopic probe used for characterization of the synthesized system showed 300-fold fluorescence enhancement for the detection of Fe3+ with a 1:1 stoichiometry in EtOH/H2O solution (2:1, v/v, HEPES buffer, 1 mM, pH 7.30). Upon addition of Fe3+ in aqueous ethanol, the probe displayed a significant fluorescence enhancement and a distinct color change (colorless to pink) that can be detected by the naked eye. The binding constant between the probe and Fe3+ was determined to be 1.16 × 104 M?1 and the corresponding detection limit was calculated to be 0.256 µM. In addition, the energy gaps between the HOMO and LUMO in RBTM and RBTM-Fe3+ were calculated using DFT calculations to be 92.93 kcal/mol and 37.49 kcal/mol, respectively. The results indicate that binding of Fe3+ to RBTM lowered the HOMO–LUMO energy gap of the complex and stabilized the system. Fluorescence imaging experiments demonstrated that RBTM can be used as a fluorescent probe to detect Fe3+ in MKN-45 cells and dorsal root ganglia, thus revealing that RBTM could be used for biological applications. |
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| Keywords: | Rhodamine B Fe(III) ions Fluorescent chemosensors MKN-45 cells Dorsal root ganglia |
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