A novel fluorescent probe based on naphthalimide for imaging nitroreductase (NTR) in bacteria and cells |
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| Affiliation: | 1. Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Xinong Road 22, Yangling, Shaanxi 712100, PR China;2. Zhejiang Runtu Co., Ltd, No. 1 Building of Wealth Square, No. 1009 Shimin Avenue Shangyu City, Zhejiang 312368, PR China;3. State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian 116023, PR China;1. Research & Development Microbiology, bioMérieux SA, 3 route de Port Michaud, 38 390 La-Balme-les-Grottes, France;2. Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK;3. École Nationale Supérieure de Chimie de Paris, 11 rue Pierre et Marie Curie, F75231 Paris Cedex 05, France;4. Department of Microbiology, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK;1. Department of Radiology, Stanford, CA, 94305, USA;2. Department of Radiation Oncology, Stanford University, Stanford, CA, 94305, USA;1. the United Innovation of Mengchao Hepatobiliary Technology Key Laboratory of Fujian Province, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou, 350025, PR China;2. Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, PR China;3. Liver Disease Center, The First Affiliated Hospital of Fujian Medical University, Fuzhou, 350005, PR China;4. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, PR China;5. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350116, PR China;1. College of Pharmacy and Research Institute of Life and Pharmaceutical Sciences, Sunchon National University, Suncheon 57922, Republic of Korea;2. Department of Fundamental Pharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea |
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| Abstract: | A nitroreductase (NTR) responsive fluorescent probe, Na-NO2, comprising p-nitrobenzyl as the unique recognition group and 1,8-naphthalimide as fluorophore, was synthesized. Na-NO2 showed remarkable fluorescence “turn-on” signal in the presence of NTR under DMSO/H2O (1:19, v/v) buffered with PBS (pH = 7) solution in the presence of NADH (300 µM). Furthermore, the probe has a low detection limit down to 3.4 ng/mL and it is very sensitive towards the NTR in Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), normal and tumor cells such as HL-7702, HepG-2 and MCF-7. |
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| Keywords: | 1,8-Naphthalimide Escherichia coli (E. coli) Staphylococcus aureus. (S. aureus) |
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