Cross talk between hedgehog and bone morphogenetic proteins occurs during cardiomyogenesis in P19 cells |
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Authors: | Peter Junior Gianakopoulos Ilona S Skerjanc |
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Institution: | (1) Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, 451 Smyth Road, Ottawa, Ontario, Canada, K1H 8M5;(2) Department of Biochemistry, Medical Sciences Building, University of Western Ontario, London, Ontario, Canada, N6A 5C1; |
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Abstract: | Hedgehog (Hh) signaling plays a role in heart morphogenesis and can initiate cardiomyogenesis in P19 cells. To determine if
Hh signaling is essential for P19 cell cardiomyogenesis, we determined which Hh factors are expressed and the effect of Hh
signal transduction inhibitors. Here, we find that the Hh gene family and their downstream mediators are expressed during
cardiomyogenesis but an active Hh signaling pathway is not essential. However, loss of Hh signaling resulted in a delay of
BMP-4, GATA-4, Gli2, and Meox1 expression during cardiomyogenesis. By using Noggin-overexpressing P19 cells, we determined
that Hh signaling was not active during Noggin-mediated inhibition of cardiomyogenesis. Thus, there is cross talk between
the Hh and BMP signaling pathways and the Hh pathway appears important for timely cardiomyogenesis. |
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