Active NDH-1 complexes from the cyanobacterium Synechocystis sp. strain PCC 6803 |
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| Authors: | Ma Weimin Deng Young Ogawa Teruo Mi Hualing |
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| Affiliation: | National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Graduate School of the Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, PR China |
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| Abstract: | We identified eight bands by staining native gels for NADPH-nitrobluetetrazolium oxidoreductase activity after electrophoresis ofn-dodecyl-ß-D-maltoside-treated membranes of Synechocystissp. strain PCC 6803. Among them, bands A, C, D and E were attributedto the activity of NADPH dehydrogenase (NDH-1). Band A is ahighly active supercomplex of NDH-1 (about 1,000 kDa) that wasabsent in the |
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