Affinity chromatography of extracellular cellulase from chaetomium cellulolyticum

Summary An endo--glucanase of C.cellulolyticum was purified by a procedure involving concanavalin A (Con A)-Sepharose chromatography and polyacrylamide gel electrophoresis (PAGE). The enzyme produced G1 and oligosaccharides from CMC. Chromatography on Procion Red HE3B-Agarose proved to be useful in the separation of cellobiase from cellobiose dehydrogenase.Abbreviations CMC carboxymethyl cellulose - CM-cellulase carboxymethyl cellulase - FP-cellulase filter paper degrading cellulase - G1 glucose - G2 cellobiose - G3 cellotriose - G4 cellotetraose - G5 cellopentaose - G6 cellohexaose - G7 celloheptaose - -MG methyl--D-glucoside - pNPG p-nitrophenyl--glucopyranoside - pNP p-nitrophenol - CBDH cellobiose dehydrogenase