Affinity chromatography of extracellular cellulase from chaetomium cellulolyticum |
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Authors: | P Fähnrich K Irrgang |
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Institution: | (1) Institute of Biotechnology, Nuclear Research Centre Jülich, P.O. Box 1913, D-5170 Jülich, Federal Republic Germany |
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Abstract: | Summary An endo- -glucanase of C.cellulolyticum was purified by a procedure involving concanavalin A (Con A)-Sepharose chromatography and polyacrylamide gel electrophoresis (PAGE). The enzyme produced G1 and oligosaccharides from CMC. Chromatography on Procion Red HE3B-Agarose proved to be useful in the separation of cellobiase from cellobiose dehydrogenase.Abbreviations CMC
carboxymethyl cellulose
- CM-cellulase
carboxymethyl cellulase
- FP-cellulase
filter paper degrading cellulase
- G1
glucose
- G2
cellobiose
- G3
cellotriose
- G4
cellotetraose
- G5
cellopentaose
- G6
cellohexaose
- G7
celloheptaose
- -MG
methyl- -D-glucoside
- pNPG
p-nitrophenyl- -glucopyranoside
- pNP
p-nitrophenol
- CBDH
cellobiose dehydrogenase |
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