Pleiotropic changes in Arabidopsis <Emphasis Type="Italic">f5h</Emphasis> and <Emphasis Type="Italic">sct</Emphasis> mutants revealed by large-scale gene expression and metabolite analysis |
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Authors: | Jun?Huang V-S?Bhinu Xiang?Li Zafer?Dallal Bashi Rong?Zhou Email author" target="_blank">Abdelali?HannoufaEmail author |
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Institution: | (1) Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada;(2) Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford Street, London, ON, N5V4T3, Canada; |
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Abstract: | Hydrocinnamic acid esters, lignin, flavonoids, glucosinolates, and salicylic acid protect plants against UV exposure, oxidative
stress, diseases, and herbivores. Through the phenylpropanoid pathway, certain Brassicaceae family members, including Arabidopsis thaliana and Brassica napus, accumulate large amounts of the anti-nutritive sinapoylcholine (sinapine) in the seed. We successfully down-regulated activities
of key enzymes in the pathway including F5H and SCT and achieved reduction of sinapine and lignin in B. napus seeds. Despite this success, it was unclear how multiple agronomic traits were affected in the transgenic plants. Here, we
report altered large-scale gene expression of new alleles of f5h and sct mutants of A. thaliana and resultant accumulation of sinapoylglucose, disinapoylglucose, quercetin-3-O-rhamnoside, salicylic acid glucoside, and total indolyl glucosinolates in the two mutants. Expression of several flowering
genes was altered in these mutants when grown under drought and NaCl treatments. Furthermore, both mutants were more susceptible
to fungal infection than the wild type. Microarray experiments identified distinctive spatial and temporal expression patterns
of gene clusters involved in silique/seed developmental processes and metabolite biosynthesis in these mutants. Taken together,
these findings suggest that both f5h and sct mutants exhibit major differences in accumulation of diverse metabolites in the seed and profound changes in global large-scale
gene expression, resulting in differential pleiotropic responses to environmental cues. |
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