Growth and nutritional characteristics of cowpea rhizobia |
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Authors: | Mark D Stowers G H Elkan |
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Institution: | (1) NPI, University Research Park, Salt Lake City, USA;(2) Department of Microbiology, North Carolina State University, Raleigh, USA |
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Abstract: | Summary Twenty-five slow-growing strains of cowpea rhizobia were examined for growth and nutritional characteristics. Growth and nutritional data of these isolates were surprisingly homogeneous given their proposed genetic diversity. Most strains tested were capable of anaerobic growth in the presence of nitrate and all were found capable of autotrophic growth in a defined atmosphere of CO2 and H2 with oxygen or nitrate as terminal electron acceptors. These isolates grew heterotrophically with various carbohydrates and organic acids. Nitrogen utilization was consistent with that of other slow-growing rhizobia. Medium composition strongly affected the final pH of the culture. Cowpea rhizobia generally did not require vitamins; those requiring vitamins exhibited good growth when biotin was supplemented to the medium. |
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Keywords: | Autotrophic growth Carbon nutrition Cowpea rhizobia Growth Nitrogen nutrition Rhizobia |
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