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The 1H-NMR relaxation of Rhus laccase: assignment to different types of copper |
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| Authors: | A Rigo E F Orsega P Viglino L Morpurgo M T Graziani G Rotilio |
| Institution: | 1. Laboratory of Biophysics and Molecular Biology, Institute of General Pathology, University of Padua, Padua, Italy;2. Institute of Physical Chemistry, University of Venice, Venice, Italy;3. C.N.R. Center of Molecular Biology, Institute of Biological Chemistry, University of Rome, Rome, Italy |
| Abstract: | 1H-NMR relaxation measurements of Rhus laccase showed that a portion of the relaxivity was specifically abolished by less than stoichiometric EDTA. Another portion of relaxivity was removed by addition of N3(-) to the EDTA saturated enzyme. This treatment or selective removal of the Type 2 Cu left a large residual paramagnetic relaxivity (1700 M-1s-1) which was assigned to the Type 1 Cu. It is concluded that only a portion of the laccase relaxivity can be assigned to the Type 1 Cu and that this copper type does not behave homogeneously: the two fractions have different relaxivity, 5200 and less than or equal to 2400 M-1s-1 respectively. |
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