Investigation of electrophysiological responses of Neurospora crassa to blue light |
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Authors: | T V Potapova N N Levina T A Belozerskaya M S Kritsky L M Chailakhian |
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Institution: | (1) A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, M. V. Lomonosov State University, Moscow, USSR;(2) A. N. Bach Institute of Biochemistry, USSR Academy of Sciences, Moscow, USSR;(3) Institute for Information Transmission Problems, USSR Academy of Sciences, Moscow, USSR |
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Abstract: | The influence of light in a spectrum range of 350–500 nm 20 W m-2 (20,000 erg · cm-2 · s-1) has been studied in the mycelial cells of Neurospora crassa. Light-induced input resistance and membrane potential changes can be measured by means of intracellular microelectrodes. The value of the input resistance reached maximum after a 2–5 min illumination. The maximum hyperpolarization of the cell membrane reaching 30–40 mV was observed after 20–25 min illumination, when the input resistance values did not differ significantly in the illuminated and non-illuminated cells. |
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Keywords: | Blue light Membrane potential Input resistance Neurospora crassa |
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