Polyphasic characterization of water bloom forming Raphidiopsis species (cyanobacteria) from central China |
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Authors: | Renhui Li Steven W Wilhelm Wayne W Carmichael Makoto M Watanabe |
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Institution: | aInstitute of Hydrobiology, The Chinese Academy of Sciences, Wuhan, Hubei 430072, China;bDepartment of Microbiology, University of Tennessee, Knoxville, TN 37996, USA;cDepartment of Biological Sciences, Wright State University, Dayton, OH 45435, USA;dNational Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki 305-0053, Japan |
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Abstract: | Two strains of Raphidiopsis Fritsch et Rich were isolated from a fishpond in Wuhan city, China and rendered axenic, and characterized by a combination of morphological, physiological, biochemical and genetic methods. Morphologically the strains were identified as Raphidiopsis mediterranea Skuja (straight trichomes) and R. curvata Fritsch et Rich (coiled trichomes). These two strains demonstrated slight differences in optimal temperature range and GC content, while sharing some common characteristics including inability to grow hetertrophically, similar salinity tolerance (up to 0.78%) and an identical fatty acid composition. Cyanotoxins were not found in the strain of R. mediterranea, however, the strain of R. curvata contained both deoxy-cylindrospermopsin and cylindrospermopsin. Phylogenetic affiliations inferred from 16S rRNA gene sequences demonstrated that both Raphidiopsis strains clustered with Cylindrospermopsis, demonstrating their phylogenetic ties to Nostocaceae. |
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Keywords: | Characterization Cyanobacteria Cyanotoxins Phylogeny Planktonic Raphidiopsis |
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