Changes in Protein Composition and the H+-ATPase Activity of the Plasma Membrane during Induction of Callus from Tuber Tissues of Jerusalem Artichoke |
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Authors: | Hase Akira |
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Institution: | Biological Laboratory, Hakodate College, Hokkaido University of Education Hakodate, 040 Japan |
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Abstract: | Changes in composition and synthesis of the proteins in plasmamembranes during early periods of induction of callus from tubertissues of Jerusalem artichoke were examined in relation toanalogous changes in H+-ATPase activity. By the 12th h of culture,vanadate-sensitive ATPase activity had increased more than 3.5-fold.The level of a polypeptide with a molecular mass of 97 kDa,which putatively corresponded to a subunit of the plasma membraneH+-ATPase, also showed a similar increase. Increases in ATPaseactivity and in the level of the 97-kDa polypeptide occurredindependently of the presence of 2,4-D in the culture mediumbut the rate of increase in both cases was slightly higher fortissue disks cultured with 2,4-D than for the control disksin medium without 2,4-D for the first 12 h of culture. The increasein the level of the 97-kDa polypeptide may be ascribed predominantlyto synthesis de novo during the early period of culture. Enhancedsynthesis of the 97-kDa polypeptide in the cultured tissuesmay have resulted in the increases in ATPase activity. Sinceauxin itself may stimulate H+-ATPase activity, the activatedH+-ATPase may be further stimulated in tissue disks culturedwith 2,4-D. The H+-ATPase activated in this way may produceconditions that facilitate the induction of callus from tubertissues of Jerusalem artichoke during the early period of culture. (Received July 13, 1992; Accepted October 19, 1992) |
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