A sensitive method for the separation and quantitation of (14C)proline and (14C)hydroxyproline from the collagen of rat uterus |
| |
Authors: | R A Salvador I Tsai |
| |
Affiliation: | Research Division, Hoffmann-La Roche Inc., Nutley, New Jersey 07110 USA |
| |
Abstract: | A specific and sensitive method is described for the isolation and quantitation of [14C]proline and [14C]hydroxyproline from uterine collagen of the immature rat. Selectivity is achieved in this isolation by using a protease-free bacterial collagenase. There is complete release of hydroxyproline from uterine protein if the latter is suspended by sonication prior to treatment with collagenase. There is a consistent recovery of [14C]proline and [14C]hydroxyproline when they are added to protein hydrolysates of uterus and then subjected to the procedures required for their isolation and quantitation. It is possible using this method to determine the incorporation of [14C]proline into collagen of the rat uterus and to quantitate its conversion to [14C]hydroxyproline. Coupled with the colorimetric methods for proline and hydroxyproline, it is also possible to determine their specific activity. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|