用体内叶绿素a荧光诱导动力学鉴定番茄的抗冷性

研究了冷害温度对具有不同抗冷性品种的番茄叶片的体内叶绿素a荧光诱导动力曲线的影响。实验结果指出，在低温处理(8℃，5℃，2℃下，暗中24小时)后，番茄叶片的体内叶绿素a荧光诱导动力学曲线有了明显的改变，Fv／Fo值、Rfd值降低了，光系统II原初光能转换效率和潜在的光合活力均受到抑制。我们在苗期和开花期得到的实验结果均表明，在番茄叶片的叶绿素a荧光诱导动力学曲线和这些荧光参数改变的程度与该品种的已知抗冷性之间呈现较好的相关性。我们认为，体内叶绿素a荧光诱导动力学方法是鉴定番茄抗冷性的一个快速、灵敏和可靠的方法，并可用于其他绿色植物的抗冷性鉴定中。

In Vivo Chlorophyll a Fluorescence Induction Kinetics as a Tool for Chilling-Tolerance Detection in Tomato

The effects of chilling temperature on in vivo chlorophyll a fluorescence induction kinetic curves of tomato leaves from several varieties with different chilling-tolerance had been studied. The experimental results indicated that, after chilling treatments, chlorophyll a fluorescence induction kinetic curves of tomato leaves were changed obviously,the Fv/Fo and Rfd values were decreased,and the primary light energy conversion of photosystem II and the potential photosynthetic activity had been inhibited. Our results obtained both at seedling and blooming stages showed that, there were good relationships between the degree of changes of the fluorescence curves, the two fluorescence parameters of tomato leaves and the known order of chilling-tolerance of these tomato varieties. It is considered that, in vivo chlorophyll a fluorescence induction kinetics method is a sensitive, rapid and reliable tool for chilling-tolerance detection in tomato, and this method could be applied to chilling-tolerance detection in other green plants.