丛枝菌根真菌与根瘤菌互作及类黄酮对互作效果的影响

研究了中华根瘤菌与AM真菌互作或加入适量的类黄酮(150nmol·L^-1和1．5μmol·L^-1)对紫云英的生物量、结瘤数、AM真菌的侵染率、菌丝ALP和SDH酶活性的影响．结果表明，与对照组0、AMF、Rh]相比，Rh+AMF组紫云英的生物量、结瘤数、AM真菌侵染率和菌丝酶活都有显著差异；与对照组及Rh+AMF组相比，类黄酮处理组紫云英的生物量、结瘤数、AM真菌侵染率和菌丝酶活差异更加显著，但不同类黄酮间(apigenin和hesperitin)，或同一种类黄酮不同浓度处理问(150nmol·L^-1和1．5umol·L^-1)差异不明显，说明AM真菌与根瘤菌互作对紫云英的结瘤固氮、AM真菌的侵染和菌丝的生长都有促进作用．当Rh+AMF组加入适量的类黄酮时。这种促进作用更加显著．

Interaction between AM fungi and Rhizobium and effects of flavonoids on it

Study showed that the biomass and nodule number of Astragalus sinicus, AM tungal infection rate, and hyphal enzyme activity of ALP and SDH were significant influenced by Rhizobium, AM fungi and flavonoids. Rh + AMF treatments had obvious differences with control in the biomass and nodule numbers of Astragalus sinicus, AM fungal infection rate and hyphal enzyme activity; and flavonoid treatments had more obvious differences with Rh + AMF treatments and control in these aspects, but no obvious differences were found in different flavonoids (apigenin and hesperitin) or in different concentrations (150 nmol x L(-1) and 1.5 micromol x L(-1)) of the same flavonoid. The results showed that nodule-generation and nitrogen-fixation of Rhizobium and AM fungi infection were obvious promoted by Rhizobium and AM fungi, and this promotion effects were more significant when an appropriate amount of flavonoids was added in the Rh + AMF treatments.