pFJ265, a new cloning vehicle for Streptomyces |
| |
| Authors: | M D Jones J A Mabe W M Nakatsukasa J T Fayerman |
| |
| Affiliation: | Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285 USA |
| |
| Abstract: | A 9.3-kb plasmid, pNM100, was isolated from Streptomyces virginiae (NRRL 15156) and characterized. Streptomyces genes for thiostrepton and neomycin resistance were cloned into pNM100 to yield a small plasmid derivative, pFJ265, that is suitable for Streptomyces gene cloning. pFJ265 is a 9.2-kb nonconjugative plasmid and has a copy number of several hundred per chromosome. |
| |
| Keywords: | To whom reprint requests should be addressed. |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
