Rapid identification of Acremonium lolii and Acremonium coenophialum endophytes through arbitrarily primed PCR |
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| Authors: | D Liua R van Heeswijck G Latch T Leonforte M Panaccio C Langford P Cunningham K Reed |
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| Institution: | Victorian Institute of Animal Science, Attwood, Victoria 3049, Australia; Department of Horticulture, Viticulture and Oenology, University of Adelaide, Adelaide, South Australia 5000, Australia; AgResearch Grasslands, Palmerston North, New Zealand; Pastoral and Veterinary Institute, Hamilton, Victoria 3300, Australia |
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| Abstract: | Abstract Using a random decamer 5'-CCGAGGTGAC-3' in an arbitrarily primed PCR, similar band patterns were observed between Acremonium lolii and A. coenophialum DNA, which were somewhat different from those formed by other fungal DNA. Despite sharing bands of around 0.7, 0.9 and 2.1 kb, A. lolii can be distinguished from A. coenophialum by the presence of an additional band at around 0.5 kb in the arbitrarily primed PCR. |
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| Keywords: | Acremonium lolii Acremonium coenophialum Endophyte Identification Arbitrarily primed PCR |
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