树鼩(Tupaia belangeri)淋巴细胞的自体花结

参与人自体花结(A花结)形成的分子(如CD2/LFA-3),与免疫细胞的粘附和激活有关。我们曾发现,人和猴淋巴细胞表面的树鼩红细胞(TRBC)受体不同于绵羊红细胞(SRBC)受体(CD2),可能是一种新的白细胞分化抗原。花结试验表明,树鼩的外周血淋巴细胞(TPBL)和胸腺细胞都能形成A花结,结花率分别为20.9％和11.1％;而绵羊红细胞花结(E花结)形成率分别是20.9％和1.1％。以四种单克隆抗体(McAb)(Leu 5,0-275,AICD2.1和E2 McAb)进行树鼩A花结和E花结的抑制与抗原调变试验,结果表明,这些抗体对树鼩的A花结都没有明显的抑制或调变作用,但对E花结的抑制及调变作用明显。说明TPBL表面的TRBC受体不同于SRBC受体,与CD2/LFA-3及E2分子无关。因此,TPBL的A花结与E花结形成机制不同。

THE ROSETTING OF TREE SHREW LYMPHOCYTES WITH ALLOGENEIC RED BLOOD CELLS

The lymphocytes from rat, mouse, hamstes, rabbit, sheep, cattle, pig, and human may form rosettes with their autologous or allogcneic red blood cells (A rosette formation). It has been found that tree shrew (Tupaia belangeri) red blood cell (TRBC) receptors on human and macaque T cells are different from E receptor (CD2). In the present study, we have demonstrated both PEL and thymocytes from tree shrew formed rosettes with TRBC at the percentages of 20.8% and 11.1%, respectively. The percentages of E rosette were 20.9% in PEL and 1.1% in thymocytes. The numbers of RBC binding to individual PEL or thymocyte were counted and analysed. In order to approach the correlation between TRBC and SRBC receptors on lymphocytes of tree shrew, and to investigate the mechanism of A rosette and E rosette formation of tree shrew PBL, rosette inhibition assay and antigenic modulation were performed with CD2 McAbs (Leu 5, T088, T091) and E2 McAb. A rosette formation of tree shrew PBL was neither inhibited nor modulated by these McAbs. On the contrary, the E rosette formation was obviously inhibited and modulated by the same McAbs. The results suggest that TRBC receptor on tree shrew PBL is also different from E receptor, and A rosette formation of tree shrew PBL is not dependent on CD2, LFA-3 and E2 molecules which are necessary for E rosette.