Trypanosoma brucei: recognition in vitro of two developmental forms by murine macrophages |
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Authors: | D M Mosser J F Roberts |
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Institution: | Physiology Department, North Carolina State University, Raleigh, North Carolina 27650, U.S.A. |
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Abstract: | In vitro, murine macrophages attach to the midgut form of Trypanosoma brucei in the absence of exogenous proteins. Recognition appears to be specific and saturable. Attachment is mediated by a non-Fc, non-C3 receptor on the macrophage plasma membrane. The attachment mechanism is protease sensitive, temperature sensitive, requires the presence of divalent cations, and is functional in fetal calf serum-free medium. The midgut form is also lysed in normal rabbit serum by activating the alternative complement pathway. The form isolated from the blood is neither lysed in normal serum nor is it spontaneously recognized by the macrophage, in vitro. Partial trypsinization of the bloodstream form, however, results in both the triggering of alternative complement pathway lysis and spontaneous uptake into macrophages. Murine macrophages attach to the midgut form of T. brucei by a receptor on the macrophage plasma membrane which is capable of recognizing particulate activators of the alternative complement pathway. |
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Keywords: | Hemoflagellate Protozoa parasitic Macrophage mouse Phagocytosis Macrophage plasma membrane Alternative complement pathway Receptors C3b and Fc |
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