(1) Department of Biological Sciences, Kyungnam University, 630-701 Masan, Korea;(2) Department of Botany, University of Queensland, Brisbane, Australia
Abstract:
We evaluated the efficiency of callus induction and plantlet regeneration from hypocotyl explants of broccoli (Brassica oleracea var. italica). The cultivars were ‘Marathon’, ‘Greenbelt’, and ‘Shogun’. Transformation success was not affected by the presence
of tobacco feeder-cell layers on the culture media. The frequency of shoot regeneration was greater from 10-d-old hypocotyls
than from 14-d-old hypocotyls. Both ‘Marathon’ and ‘Greenbelt’ had higher potentials for tissue regeneration than did ‘Shogun’.
We found that for transformation selection, the optimum concentration was either 50 mg/L kanamycin or 100 mg/L genetkin.