Influence of pH on the formation and location of β-glucosidase inAspergillus terreus grown on cellulose |
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Authors: | J D’Souza O Volfová |
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Institution: | (1) Department of Technical Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4;(2) Present address: Centre for Postgraduate Instruction and Research, Panaji, Goa, India |
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Abstract: | Wild strainof Aspergillus terreus is very good producer of /gb-glucosidase. It produces 15 nkat/mL (0.9 IU/mL) of extracellular β-glucosidase at pH 5.0. The
medium pH level strongly affects the production and binding of β-glucosidase on the cells and on residual cellulose. At pH
4.0 the rate of enzyme synthesis and the level of total activity is highest but 60—75 % of this activity is bound. At higher
pH levels the enzyme is mainly released to the medium. |
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Keywords: | |
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