Primary structure of V-ATPase subunit B from Manduca sexta midgut. |
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| Authors: | F J Novak R Gr?f R B Waring M G Wolfersberger H Wieczorek W R Harvey |
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| Institution: | Department of Biology, Temple University, Philadelphia, PA 19122. |
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| Abstract: | The amino acid sequence of a vacuolar-type ATPase (V-ATPase) subunit B has been deduced from a cDNA clone isolated from a Manduca sexta larval midgut library. The library was screened by hybridization with a labeled cDNA encoding subunit B of Arabidopsis thaliana tonoplast V-ATPase. The M. sexta V-ATPase subunit B consists of 494 amino acids with a calculated M(r) of 54,902. The amino acid sequence deduced for V-ATPase subunit B of M. sexta is between 98% and 76% identical with that of seven other V-ATPase subunits B and greater than 52% identical with three archaebacterial ATPase subunits B. |
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