Variability in sequence of Strawberry vein banding virus |
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Authors: | D Hanzliková Va?ková J ?pak K Petrzik |
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Institution: | (1) Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, Branišovská 31, CZ-37005 České Budějovice, Czech Republic |
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Abstract: | The variability of the Strawberry vein banding virus (SVBV) isolates was investigated. In total 267 strawberry plants from 6 European countries and North America were tested
for the presence of SVBV. Only 4 plants were positive. Partial genomic sequences of the capsid protein gene of three North
American SVBV isolates were determined. Only minor sequence variability (0.7 %) was observed during a comparison with existing
nucleotide data of the European and the North American isolates (9 isolates). No variability at all could be found in the
annealing regions of primers and probes used for molecular detection of SVBV for these isolates. However, a comparison to
a sequence of a Chinese isolate published recently revealed a much higher DNA sequence difference (9.5 %) of this isolate. |
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Keywords: | caulimovirus diversity Fragaria sp NASBA PCR quarantine |
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